Hotspot

ccsA-ndhD

Genome · 116542-116842 · 301 bp

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Pi 0.0609

MarkerSeek score 74.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 301
variable_sites: 37
pi: 0.0609
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCTAATGAGGCATGGGGATC	ATGAGTGGTTTTGCTGCAGA	55.0	45.0	58.2	58.0	670-877 bp	1.0	86.9
ccsA-ndhD_p2	GGGAGCAGTATGGGCTAATG	ATGAGTGGTTTTGCTGCAGA	55.0	45.0	57.8	58.0	683-890 bp	1.0	86.8
ccsA-ndhD_p3	GCTAATGAGGCATGGGGATC	TTCTCGATGGCTTCTCTTGC	55.0	50.0	58.2	58.0	700-907 bp	1.0	86.7
ccsA-ndhD_p4	GCGCCAAGTGCTATTTGTAC	ATGAGTGGTTTTGCTGCAGA	50.0	45.0	57.8	58.0	1093-1300 bp	1.0	86.3
ccsA-ndhD_p5	GCGCCAAGTGCTATTTGTAC	TTCTCGATGGCTTCTCTTGC	50.0	50.0	57.8	58.0	1123-1330 bp	1.0	86.2