Hotspot

trnL-UAG-ccsA

Genome · 115476-115569 · 94 bp

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Pi 0.0621

MarkerSeek score 80.00

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: Genome
length_bp: 94
variable_sites: 14
pi: 0.0621
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGACGATTGCAATGATTGAA	50.0	35.0	57.9	53.9	219-226 bp	1.0	51.6
trnL-UAG-ccsA_p2	GTAGACACGCTGCTCTTAGG	TGACGATTGCAATGATTGAA	55.0	35.0	57.8	53.9	220-227 bp	1.0	51.0
trnL-UAG-ccsA_p3	AAATTGGTAGACACGCTGCT	TGACGATTGCAATGATTGAA	45.0	35.0	57.8	53.9	226-233 bp	1.0	50.9
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	TGACGATTGCAATGATTGAA	55.0	35.0	57.8	53.9	221-228 bp	1.0	50.9
trnL-UAG-ccsA_p5	TAGACACGCTGCTCTTAGGA	TGACGATTGCAATGATTGAAA	50.0	33.3	57.9	54.8	219-226 bp	1.0	50.5