Hotspot

rpl32-trnL-UAG

Genome · 114514-115395 · 882 bp

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Pi 0.0384

MarkerSeek score 78.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 882
variable_sites: 82
pi: 0.0384
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGTCAAAAAGTTTTGTTTTTATGCGA	TCCTAAGAGCAGCGTGTCTA	26.9	50.0	57.8	57.9	641-1099 bp	1.0	50.8
rpl32-trnL-UAG_p2	AGTCAAAAAGTTTTGTTTTTATGCGA	AGCAGCGTGTCTACCAATTT	26.9	45.0	57.8	57.8	634-1092 bp	1.0	50.8
rpl32-trnL-UAG_p3	AGTCAAAAAGTTTTGTTTTTATGCGA	CCTAAGAGCAGCGTGTCTAC	26.9	55.0	57.8	57.8	640-1098 bp	1.0	50.8
rpl32-trnL-UAG_p4	AGTCAAAAAGTTTTGTTTTTATGCGA	CTAAGAGCAGCGTGTCTACC	26.9	55.0	57.8	57.8	639-1097 bp	1.0	50.8
rpl32-trnL-UAG_p5	AGTCAAAAAGTTTTGTTTTTATGCGA	GAGCAGCGTGTCTACCAATT	26.9	50.0	57.8	58.3	635-1093 bp	1.0	50.8