Hotspot

rps8-rpl14

Genome · 81773-81990 · 218 bp

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Pi 0.0208

MarkerSeek score 66.10

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: Genome
length_bp: 218
variable_sites: 9
pi: 0.0208
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TGCTATTCGAACCGTTCCTT	GAACTCGAGTTTTTGGTGCG	45.0	50.0	57.2	58.0	351-440 bp	1.0	79.6
rps8-rpl14_p2	GGCAATAGTGTCCCTACCCA	GAACTCGAGTTTTTGGTGCG	55.0	50.0	58.8	58.0	291-380 bp	1.0	79.6
rps8-rpl14_p3	TCGGCAATAGTGTCCCTACC	GAACTCGAGTTTTTGGTGCG	55.0	50.0	58.9	58.0	293-382 bp	1.0	78.9
rps8-rpl14_p4	TGCTATTCGAACCGTTCCTT	ACTCGAGTTTTTGGTGCGAT	45.0	45.0	57.2	58.1	349-438 bp	1.0	78.8
rps8-rpl14_p5	GGCAATAGTGTCCCTACCCA	ACTCGAGTTTTTGGTGCGAT	55.0	45.0	58.8	58.1	289-378 bp	1.0	78.8