Hotspot

petA-psbJ

Genome · 64631-65239 · 609 bp

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Pi 0.018

MarkerSeek score 67.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 609
variable_sites: 21
pi: 0.018
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTTCTTCTTGGCAGCTGTT	GGCTCATATTCTGGGTTGGG	45.0	55.0	57.9	58.0	737-952 bp	1.0	83.0
petA-psbJ_p2	TTGTTCTTCTTGGCAGCTGT	GGCTCATATTCTGGGTTGGG	45.0	55.0	57.9	58.0	738-953 bp	1.0	83.0
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	GGCTCATATTCTGGGTTGGG	50.0	55.0	58.2	58.0	675-890 bp	1.0	82.3
petA-psbJ_p4	GGGCTTTTGTTCTTCTTGGC	GGCTCATATTCTGGGTTGGG	50.0	55.0	57.8	58.0	744-959 bp	1.0	82.2
petA-psbJ_p5	TGTTCTTCTTGGCAGCTGTT	TGATCGGTTCAATGGGCATT	45.0	45.0	57.9	57.9	766-981 bp	1.0	81.8