Hotspot

ndhC-trnV-UAC

Genome · 51639-52827 · 1189 bp

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Pi 0.0186

MarkerSeek score 67.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: Genome
length_bp: 1189
variable_sites: 57
pi: 0.0186
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTGAAGCAGAAACA	TGCATGTCGGGTCTTTGAAA	36.4	45.0	56.1	58.0	1023-1386 bp	1.0	56.2
ndhC-trnV-UAC_p2	TCATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	36.4	50.0	56.1	57.8	958-1321 bp	1.0	55.0
ndhC-trnV-UAC_p3	TCATATTCGTGAAGCAGAAACA	AATGCATGTCGGGTCTTTGA	36.4	45.0	56.1	57.8	1025-1388 bp	1.0	54.8
ndhC-trnV-UAC_p4	TCATATTCGTGAAGCAGAAACA	ATGCATGTCGGGTCTTTGAA	36.4	45.0	56.1	57.8	1024-1387 bp	1.0	54.8
ndhC-trnV-UAC_p5	TCATATTCGTGAAGCAGAAACA	GCATGTCGGGTCTTTGAAAC	36.4	50.0	56.1	57.7	1022-1385 bp	1.0	53.8