Hotspot

rps14-psaB

Genome · 38579-38715 · 137 bp

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Pi 0.065

MarkerSeek score 67.50

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: Genome
length_bp: 137
variable_sites: 20
pi: 0.065
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	CGGTGGGGATTGCAATTTTC	TTTCCTGGCGTGGATATTGG	50.0	50.0	58.0	57.9	498-507 bp	1.0	87.3
rps14-psaB_p2	ATGAAGACGTATGGGTGCAC	TTTCCTGGCGTGGATATTGG	50.0	50.0	58.0	57.9	525-534 bp	1.0	87.0
rps14-psaB_p3	CGGTGGGGATTGCAATTTTC	TGATATTTCGGCTTGGGACG	50.0	50.0	58.0	58.1	788-797 bp	1.0	86.5
rps14-psaB_p4	ATGAAGACGTATGGGTGCAC	TGATATTTCGGCTTGGGACG	50.0	50.0	58.0	58.1	815-824 bp	1.0	86.3
rps14-psaB_p5	GCTACCAAACTGCTCTACCC	TTTCCTGGCGTGGATATTGG	55.0	50.0	58.0	57.9	828-837 bp	1.0	86.1