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trnH-GUG-psbA

Genome · 71-314 · 244 bp

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Pi 0.0527

MarkerSeek score 78.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: Genome
length_bp: 244
variable_sites: 28
pi: 0.0527
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTCACAACTTCCCGCTAGAC	55.0	55.0	58.1	58.0	196-323 bp	1.0	90.4
trnH-GUG-psbA_p2	AATCCACTGCCTTGATCCAC	CTCACAACTTCCCGCTAGAC	50.0	55.0	57.9	58.0	209-336 bp	1.0	90.1
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CTCACAACTTCCCGCTAGAC	50.0	55.0	57.9	58.0	210-337 bp	1.0	90.0
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CTCACAACTTCCCGCTAGAC	50.0	55.0	57.9	58.0	211-338 bp	1.0	90.0
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TAGACCTAGCTGCTGTCGAA	55.0	50.0	58.1	57.9	181-308 bp	1.0	89.8