Hotspot

ycf1

SSC · 120114-125351 · 5238 bp

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Pi 0.0463

MarkerSeek score 76.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5238
variable_sites: 695
pi: 0.0463
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TATGAGCTGTTCGACAACGG	GCAGAATACCGTCACCCATT	50.0	50.0	58.0	58.0	700 bp	0.090909	43.3
ycf1_p2	TATGAGCTGTTCGACAACGG	TGGAAGAGGAAGGGAAGGAG	50.0	55.0	58.0	58.0	640 bp	0.090909	43.2
ycf1_p3	TATGAGCTGTTCGACAACGG	ATTATGGACCCGCTACCAGA	50.0	50.0	58.0	57.9	1069 bp	0.090909	42.8
ycf1_p4	TATGAGCTGTTCGACAACGG	GGCCCTCCTATCTCTTCCTT	50.0	55.0	58.0	57.9	1291 bp	0.090909	42.7
ycf1_p5	TATGAGCTGTTCGACAACGG	TATGGACCCGCTACCAGAAA	50.0	50.0	58.0	58.1	1067 bp	0.090909	42.5