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rps15-ycf1

SSC · 119711-120113 · 403 bp

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Pi 0.0684

MarkerSeek score 76.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 403
variable_sites: 73
pi: 0.0684
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	GCTTCCTGCAACGCTATTTG	50.0	50.0	58.0	58.1	1706-1850 bp	1.0	91.1
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	AACTCAATCGCTTCCTGCAA	50.0	45.0	58.0	58.1	1715-1859 bp	0.909091	87.4
rps15-ycf1_p3	GACCCCGTGTTTTCTTCCTT	GCTTCCTGCAACGCTATTTG	50.0	50.0	58.0	58.1	1186-1204 bp	0.636364	74.4
rps15-ycf1_p4	TGGCATCGCGTTTCAGATTA	GCTTCCTGCAACGCTATTTG	45.0	50.0	58.0	58.1	1414-1431 bp	0.272727	56.9
rps15-ycf1_p5	TGGCATCGCGTTTCAGATTA	AACTCAATCGCTTCCTGCAA	45.0	45.0	58.0	58.1	1423-1439 bp	0.181818	53.1