Hotspot

rpl32-trnL (UAG)

SSC · 110062-110607 · 546 bp

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Pi 0.0607

MarkerSeek score 77.70

Primer available yes

Label rpl32-trnL (UAG)

Feature Metadata

label_name: rpl32-trnL (UAG)
feature_type: igs
region: SSC
length_bp: 546
variable_sites: 83
pi: 0.0607
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL (UAG)_p1	GGGATATTGGGCAGCGTTAA	GTAGCAAAACCCTGGGTCAA	50.0	50.0	58.0	58.0	1143-1150 bp	0.272727	58.2
rpl32-trnL (UAG)_p2	TGGAAGCAGAACGAGAGGTA	GTAGCAAAACCCTGGGTCAA	50.0	50.0	58.1	58.0	1477-1656 bp	0.272727	56.3
rpl32-trnL (UAG)_p3	TCGGAAAAAGAAGAAGCCCC	GTAGCAAAACCCTGGGTCAA	50.0	50.0	58.1	58.0	1517-1696 bp	0.272727	56.1
rpl32-trnL (UAG)_p4	GGGATATTGGGCAGCGTTAA	AGGCCTGAAGTAGCAAAACC	50.0	50.0	58.0	58.1	1152 bp	0.090909	42.7
rpl32-trnL (UAG)_p5	GGGATATTGGGCAGCGTTAA	GCCTGAAGTAGCAAAACCCT	50.0	50.0	58.0	58.1	1150 bp	0.090909	42.7