Hotspot

ndhC-trnV (UAC)

LSC · 49288-50252 · 965 bp

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Pi 0.0501

MarkerSeek score 82.80

Primer available yes

Label ndhC-trnV (UAC)

Feature Metadata

label_name: ndhC-trnV (UAC)
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 138
pi: 0.0501
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV (UAC)_p1	AGGGGGCCATTTTCATTGTT	AGAGCACCTCGTTTACACAC	45.0	50.0	58.0	57.8	2097-2324 bp	1.0	90.0
ndhC-trnV (UAC)_p2	AGGGGGCCATTTTCATTGTT	CATCACAAGCACTAAGGGCT	45.0	50.0	58.0	57.9	2131-2315 bp	0.818182	81.4
ndhC-trnV (UAC)_p3	CTAAGGAGGGGGCCATTTTC	CATCACAAGCACTAAGGGCT	55.0	50.0	57.9	57.9	2137-2321 bp	0.818182	81.1
ndhC-trnV (UAC)_p4	AGGGGGCCATTTTCATTGTT	GTGCCAAACAGACTCCATCA	45.0	50.0	58.0	58.1	2109-2123 bp	0.272727	53.9
ndhC-trnV (UAC)_p5	CTAAGGAGGGGGCCATTTTC	GTGCCAAACAGACTCCATCA	55.0	50.0	57.9	58.1	2115-2129 bp	0.272727	53.6