Hotspot

trnT (UGU)-trnL (UAA)

LSC · 45696-46398 · 703 bp

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Pi 0.0569

MarkerSeek score 86.00

Primer available yes

Label trnT (UGU)-trnL (UAA)

Feature Metadata

label_name: trnT (UGU)-trnL (UAA)
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 107
pi: 0.0569
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT (UGU)-trnL (UAA)_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	528-810 bp	1.0	89.0
trnT (UGU)-trnL (UAA)_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	560-842 bp	1.0	88.1
trnT (UGU)-trnL (UAA)_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	522-804 bp	1.0	87.7
trnT (UGU)-trnL (UAA)_p4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	560-842 bp	1.0	86.9
trnT (UGU)-trnL (UAA)_p5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	554-836 bp	1.0	86.8