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petN-psbM

LSC · 28237-29196 · 960 bp

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Pi 0.0527

MarkerSeek score 82.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 960
variable_sites: 137
pi: 0.0527
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGTTCTCATTCCCCTGGGA	ATTGATTGGGCCAGGAATCC	50.0	50.0	58.0	57.9	1366-1634 bp	0.181818	53.5
petN-psbM_p2	TGTTCTCATTCCCCTGGGAT	ATTGATTGGGCCAGGAATCC	50.0	50.0	58.0	57.9	1365-1633 bp	0.181818	53.5
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	ATTGATTGGGCCAGGAATCC	50.0	50.0	58.0	57.9	1086-1333 bp	0.181818	53.3
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	ATTGATTGGGCCAGGAATCC	50.0	50.0	58.0	57.9	1084-1331 bp	0.181818	53.3
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	GATTGGGCCAGGAATCCAAA	50.0	50.0	58.0	58.1	1081-1328 bp	0.181818	53.0