Hotspot

trnC (GCA)-petN

LSC · 27447-28146 · 700 bp

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Pi 0.0589
MarkerSeek score 85.90
Primer available yes
Label trnC (GCA)-petN

Feature Metadata

label_name
trnC (GCA)-petN
feature_type
igs
region
LSC
length_bp
700
variable_sites
108
pi
0.0589
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.92
markerseek_score
85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC (GCA)-petN_p1 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 492-810 bp 1.0 91.3
trnC (GCA)-petN_p2 TTTCCCCAGTTCAAATCCGG TTCTTCCCCACACTACGAGT 50.0 50.0 58.1 58.0 490-808 bp 1.0 91.3
trnC (GCA)-petN_p3 TTTCCCCAGTTCAAATCCGG CACTTCTTCCCCACACTACG 50.0 55.0 58.1 57.9 493-811 bp 1.0 90.7
trnC (GCA)-petN_p4 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 438-756 bp 1.0 90.1
trnC (GCA)-petN_p5 GGGGGACTGCAAATCCTTTT TTCTTCCCCACACTACGAGT 50.0 50.0 58.4 58.0 508-826 bp 1.0 89.1