Hotspot

atpF-atpH

LSC · 12481-12887 · 407 bp

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Pi 0.0436

MarkerSeek score 84.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 52
pi: 0.0436
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AAATCCGAAACTCCTGGCAA	CCGAGGCAGAGGGAAAAATA	45.0	50.0	57.7	57.3	415-601 bp	1.0	83.5
atpF-atpH_p2	AATCCGAAACTCCTGGCAAA	CCGAGGCAGAGGGAAAAATA	45.0	50.0	57.7	57.3	414-600 bp	1.0	83.5
atpF-atpH_p3	TCCGAAACTCCTGGCAAATG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.5	57.3	412-598 bp	1.0	82.1
atpF-atpH_p4	ATCCGAAACTCCTGGCAAAT	CCGAGGCAGAGGGAAAAATA	45.0	50.0	57.5	57.3	413-599 bp	1.0	81.7
atpF-atpH_p5	AATCCGAAACTCCTGGCAAA	GCAGAGGGAAAAATACGAGGT	45.0	47.6	57.7	58.0	409-595 bp	1.0	81.2