Hotspot

trnG (UCC)-trnR (UCU)

LSC · 9289-9452 · 164 bp

Back to Justicia

Pi 0.0614

MarkerSeek score 81.70

Primer available yes

Label trnG (UCC)-trnR (UCU)

Feature Metadata

label_name: trnG (UCC)-trnR (UCU)
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 30
pi: 0.0614
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG (UCC)-trnR (UCU)_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	225-300 bp	1.0	75.3
trnG (UCC)-trnR (UCU)_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	228-303 bp	1.0	75.1
trnG (UCC)-trnR (UCU)_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	231-306 bp	1.0	74.7
trnG (UCC)-trnR (UCU)_p4	AGCCTTCCAAGCTAACGATG	CATTAGACAATGGACGCAGT	50.0	45.0	58.0	55.9	206-281 bp	1.0	74.6
trnG (UCC)-trnR (UCU)_p5	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	234-309 bp	1.0	74.5