Hotspot

trnS (GCU)-trnG (UCC)

LSC · 7870-8543 · 674 bp

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Pi 0.0572
MarkerSeek score 90.50
Primer available yes
Label trnS (GCU)-trnG (UCC)

Feature Metadata

label_name
trnS (GCU)-trnG (UCC)
feature_type
igs
region
LSC
length_bp
674
variable_sites
115
pi
0.0572
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
90.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS (GCU)-trnG (UCC)_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 628-771 bp 1.0 63.7
trnS (GCU)-trnG (UCC)_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 627-770 bp 1.0 63.7
trnS (GCU)-trnG (UCC)_p3 ACGGAAAGAGAGGGATTCGA ACGAATCACACTTTTACCACT 50.0 38.1 58.2 55.5 682-825 bp 1.0 62.5
trnS (GCU)-trnG (UCC)_p4 CAATCCGACGCTTTAGTCCA GGAATATGACCGAAAGAACCT 50.0 42.9 58.0 55.4 670-813 bp 0.909091 59.4
trnS (GCU)-trnG (UCC)_p5 AATCCGACGCTTTAGTCCAC GGAATATGACCGAAAGAACCT 50.0 42.9 58.0 55.4 669-812 bp 0.909091 59.4