Hotspot

trnL-UAG-rpl32

SSC · 122927-123807 · 881 bp

Back to Jurinea

Pi 0.00814
MarkerSeek score 63.90
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
881
variable_sites
13
pi
0.00814
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 1008-1016 bp 1.0 79.1
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 1009-1017 bp 1.0 79.1
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 991-999 bp 1.0 78.9
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 992-1000 bp 1.0 78.9
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA AAGGAAAGGATATTGGGCGG 50.0 50.0 57.9 57.6 1018-1026 bp 1.0 78.1