Hotspot

accD-psaI

LSC · 57955-58620 · 666 bp

Back to Jurinea

Pi 0.00526

MarkerSeek score 57.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 7
pi: 0.00526
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	845-858 bp	1.0	66.9
accD-psaI_p2	CCAGAAGGGCTTATTCGATCT	AAAGAAGCCATTGCGATTGC	47.6	45.0	57.2	58.0	844-857 bp	1.0	66.9
accD-psaI_p3	TTCCAGAAGGGCTTATTCGA	AAAGAAGCCATTGCGATTGC	45.0	45.0	56.2	58.0	846-859 bp	1.0	66.9
accD-psaI_p4	TCCAGAAGGGCTTATTCGATC	CCGGAAAGACTAGGCCTACT	47.6	55.0	57.2	58.2	826-839 bp	1.0	65.2
accD-psaI_p5	CCAGAAGGGCTTATTCGATCT	CCGGAAAGACTAGGCCTACT	47.6	55.0	57.2	58.2	825-838 bp	1.0	65.1