Hotspot

ndhC-trnV-UAC

LSC · 49728-50865 · 1138 bp

Back to Jurinea

Pi 0.00484

MarkerSeek score 53.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 10
pi: 0.00484
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGCTAATCGGAGCT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1189-1286 bp	1.0	81.3
ndhC-trnV-UAC_p2	CGGTCCTTTGCTAATCGGAG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.4	58.1	1191-1288 bp	1.0	80.4
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.4	58.1	1194-1291 bp	1.0	80.4
ndhC-trnV-UAC_p4	GGTCCTTTGCTAATCGGAGC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.7	58.1	1190-1287 bp	1.0	78.2
ndhC-trnV-UAC_p5	GTCCTTTGCTAATCGGAGCT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	1174-1271 bp	1.0	76.7