Hotspot

ycf3-trnS-GGA

LSC · 43531-44442 · 912 bp

Back to Jurinea

Pi 0.00605

MarkerSeek score 38.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 912
variable_sites: 11
pi: 0.00605
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	1022-1044 bp	1.0	71.5
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	972-994 bp	1.0	71.3
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	47.6	55.0	58.0	57.8	1010-1032 bp	1.0	71.0
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	974-996 bp	1.0	70.7
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	47.6	50.0	58.0	57.6	1000-1022 bp	1.0	69.6