Hotspot

atpI-atpH

LSC · 24609-25744 · 1136 bp

Back to Jurinea

Pi 0.00397

MarkerSeek score 62.60

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 9
pi: 0.00397
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.3	58.0	1260-1272 bp	1.0	78.9
atpI-atpH_p2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	50.0	50.0	58.3	57.9	1316-1328 bp	1.0	78.3
atpI-atpH_p3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	50.0	45.0	58.3	57.9	1276-1288 bp	1.0	78.1
atpI-atpH_p4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	50.0	45.0	58.3	58.2	1261-1273 bp	1.0	77.8
atpI-atpH_p5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	50.0	55.0	58.3	58.2	1320-1332 bp	1.0	77.7