Hotspot

accD

Genome · 1771-4539 · 2769 bp

Back to Juniperus

Pi 0.0189

MarkerSeek score 58.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 2769
variable_sites: 139
pi: 0.0189
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCTATTCTAGCTCGCACCCA	TGGCAAATTCGTAACTAGACCA	50.0	40.9	57.9	57.7	2823-2934 bp	0.888889	64.7
accD_p2	TGGGGTCAGTGGATCAGTAG	TGGCAAATTCGTAACTAGACCA	55.0	40.9	58.1	57.7	2873-2984 bp	0.888889	64.1
accD_p3	GGGGTCAGTGGATCAGTAGA	TGGCAAATTCGTAACTAGACCA	55.0	40.9	57.8	57.7	2872-2983 bp	0.888889	63.9
accD_p4	ATTCTAGCTCGCACCCAAAC	TGGCAAATTCGTAACTAGACCA	50.0	40.9	58.3	57.7	2820-2931 bp	0.888889	63.1
accD_p5	ATGGGGTCAGTGGATCAGTA	TGGCAAATTCGTAACTAGACCA	50.0	40.9	57.4	57.7	2874-2985 bp	0.888889	60.6