Hotspot

atpF-atpA

Genome · 106019-106182 · 164 bp

Back to Juniperus

Pi 0.0504

MarkerSeek score 80.10

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: Genome
length_bp: 164
variable_sites: 22
pi: 0.0504
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	TATCGACTTGCTCCTAGCCA	ACTGATTTCATCGGGTCGAA	50.0	45.0	57.9	56.9	228-285 bp	1.0	82.3
atpF-atpA_p2	TATCGACTTGCTCCTAGCCA	AGCAATGCCATCTCCTACTTG	50.0	47.6	57.9	58.1	318-375 bp	1.0	80.2
atpF-atpA_p3	TATCGACTTGCTCCTAGCCA	GCAATGCCATCTCCTACTTGA	50.0	47.6	57.9	58.1	317-374 bp	1.0	80.1
atpF-atpA_p4	TATCGACTTGCTCCTAGCCA	AGCAATGCCATCTCCTACTT	50.0	45.0	57.9	56.6	318-375 bp	1.0	77.4
atpF-atpA_p5	TATCGACTTGCTCCTAGCCA	GCAATGCCATCTCCTACTTG	50.0	50.0	57.9	56.6	317-374 bp	1.0	77.3