Hotspot

psbM-petN

Genome · 90354-91045 · 692 bp

Back to Juniperus

Pi 0.0227

MarkerSeek score 68.10

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: Genome
length_bp: 692
variable_sites: 33
pi: 0.0227
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	GCACAAACGAAGCCTCAAAG	TGGGCTGCTCTAATGGTAGT	50.0	50.0	58.2	58.1	751-794 bp	1.0	84.6
psbM-petN_p2	ATTTACGCACAAACGAAGCC	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.7	58.1	757-800 bp	1.0	83.9
psbM-petN_p3	TATTCATTGCAGTGCCCACT	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.5	58.1	792-835 bp	1.0	82.2
psbM-petN_p4	CGTCATTTACGCACAAACGA	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.4	58.1	761-804 bp	1.0	81.9
psbM-petN_p5	ACGAAGCCTCAAAGTGAACT	TGGGCTGCTCTAATGGTAGT	45.0	50.0	57.4	58.1	745-788 bp	1.0	81.5