Hotspot

ycf3-psaA

Genome · 78999-79538 · 540 bp

Back to Juniperus

Pi 0.00772

MarkerSeek score 60.90

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: Genome
length_bp: 540
variable_sites: 13
pi: 0.00772
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCTGCTGAATATTGGAAGCA	TGGGTCCCTTAGCTAGTGTC	42.9	55.0	58.0	58.1	754-765 bp	1.0	72.7
ycf3-psaA_p2	AGCTGCTGAATATTGGAAGCA	GGGTCCCTTAGCTAGTGTCT	42.9	55.0	58.0	57.8	753-764 bp	1.0	72.5
ycf3-psaA_p3	AGCTGCTGAATATTGGAAGCA	TTGGGTCCCTTAGCTAGTGT	42.9	50.0	58.0	57.7	755-766 bp	1.0	71.1
ycf3-psaA_p4	TGGAAGCAAGCTATAGCACT	TGGGTCCCTTAGCTAGTGTC	45.0	55.0	56.6	58.1	741-752 bp	1.0	69.8
ycf3-psaA_p5	TGGAAGCAAGCTATAGCACT	GGGTCCCTTAGCTAGTGTCT	45.0	55.0	56.6	57.8	740-751 bp	1.0	69.6