Hotspot

rrn5-trnR-ACG

Genome · 61171-61284 · 114 bp

Back to Juniperus

Pi 0.0175

MarkerSeek score 62.80

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: Genome
length_bp: 114
variable_sites: 7
pi: 0.0175
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	224-248 bp	1.0	82.7
rrn5-trnR-ACG_p2	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	227-251 bp	1.0	82.7
rrn5-trnR-ACG_p3	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	225-249 bp	1.0	82.7
rrn5-trnR-ACG_p4	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	228-252 bp	1.0	82.6
rrn5-trnR-ACG_p5	GGGAAGCCCTGTGGAAAAAT	CCACGTGCTCTAATCCTCTG	50.0	55.0	58.4	57.8	173-197 bp	1.0	82.4