Hotspot

rrn16-trnI-GAU

Genome · 54628-55682 · 1055 bp

Back to Juniperus

Pi 0.0245

MarkerSeek score 61.30

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: Genome
length_bp: 1055
variable_sites: 61
pi: 0.0245
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	AGTGACTGGAGTGAAGTCGT	CTACCACTGAGCTAATAGCCC	50.0	52.4	58.3	57.3	821-1127 bp	1.0	73.0
rrn16-trnI-GAU_p2	AAGTCGTAACAAGGTAGCCG	CTACCACTGAGCTAATAGCCC	50.0	52.4	57.6	57.3	808-1114 bp	1.0	72.6
rrn16-trnI-GAU_p3	AGTGACTGGAGTGAAGTCGT	TACCACTGAGCTAATAGCCC	50.0	50.0	58.3	56.1	820-1126 bp	1.0	71.2
rrn16-trnI-GAU_p4	AAGTCGTAACAAGGTAGCCG	TACCACTGAGCTAATAGCCC	50.0	50.0	57.6	56.1	807-1113 bp	1.0	70.9
rrn16-trnI-GAU_p5	GGCTAGTGACTGGAGTGAAG	CTACCACTGAGCTAATAGCCC	55.0	52.4	57.3	57.3	825-1131 bp	1.0	70.1