Hotspot

trnL-CAA-ycf2

Genome · 39518-39799 · 282 bp

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Pi 0.119

MarkerSeek score 74.50

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: Genome
length_bp: 282
variable_sites: 84
pi: 0.119
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	CACGCGACACTCAAAATGTC	CGATTTCTTTGGGATCCCGT	50.0	50.0	58.1	58.0	901-1123 bp	1.0	91.2
trnL-CAA-ycf2_p2	GACACGCGACACTCAAAATG	CGATTTCTTTGGGATCCCGT	50.0	50.0	58.1	58.0	903-1125 bp	1.0	91.2
trnL-CAA-ycf2_p3	AGACACGCGACACTCAAAAT	CGATTTCTTTGGGATCCCGT	45.0	50.0	58.1	58.0	904-1126 bp	1.0	90.6
trnL-CAA-ycf2_p4	ATATGTGTCCTACCTGCCGA	CGATTTCTTTGGGATCCCGT	50.0	50.0	57.9	58.0	1207-1622 bp	0.777778	79.9
trnL-CAA-ycf2_p5	TACCTGCCGAATCTCCTCAA	CGATTTCTTTGGGATCCCGT	50.0	50.0	58.1	58.0	1197-1612 bp	0.777778	79.5