Hotspot

clpP-trnL-UAG

Genome · 29686-29818 · 133 bp

Back to Juniperus

Pi 0.0351

MarkerSeek score 70.60

Primer available yes

Label clpP-trnL-UAG

Feature Metadata

label_name: clpP-trnL-UAG
feature_type: igs
region: Genome
length_bp: 133
variable_sites: 12
pi: 0.0351
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-trnL-UAG_p1	ACCAAACTGCGTCATACTCC	TACCAATTTCACCATGGCGG	50.0	50.0	57.9	58.5	169-181 bp	1.0	84.3
clpP-trnL-UAG_p2	ACCAAACTGCGTCATACTCC	GATTCGAACCGAGATGCTCT	50.0	50.0	57.9	57.5	216-228 bp	1.0	83.5
clpP-trnL-UAG_p3	ACCAAACTGCGTCATACTCC	GGATTCGAACCGAGATGCTC	50.0	55.0	57.9	58.5	217-229 bp	1.0	83.4
clpP-trnL-UAG_p4	GGTTTCGGAGCAGAAGGATT	GATTCGAACCGAGATGCTCT	50.0	50.0	57.9	57.5	273-285 bp	1.0	82.5
clpP-trnL-UAG_p5	GGTTTCGGAGCAGAAGGATT	GGATTCGAACCGAGATGCTC	50.0	55.0	57.9	58.5	274-286 bp	1.0	82.4