Hotspot

accD-rpl23

Genome · 4540-5416 · 877 bp

Back to Juniperus

Pi 0.0206

MarkerSeek score 61.60

Primer available yes

Label accD-rpl23

Feature Metadata

label_name: accD-rpl23
feature_type: igs
region: Genome
length_bp: 877
variable_sites: 50
pi: 0.0206
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-rpl23_p1	TTCTCGTATGTGCTTCTGGC	ACGATACAGACGCTTGTGAC	50.0	50.0	58.0	58.0	1731-1787 bp	1.0	86.5
accD-rpl23_p2	AGTATGGGATCGGTAGTGGG	ACGATACAGACGCTTGTGAC	55.0	50.0	58.0	58.0	1804-1860 bp	1.0	86.5
accD-rpl23_p3	TTCTCGTATGTGCTTCTGGC	TGACGATACAGACGCTTGTG	50.0	50.0	58.0	58.0	1733-1789 bp	1.0	86.5
accD-rpl23_p4	AGTATGGGATCGGTAGTGGG	TGACGATACAGACGCTTGTG	55.0	50.0	58.0	58.0	1806-1862 bp	1.0	86.5
accD-rpl23_p5	GTTCATGGGAGGCAGTATGG	ACGATACAGACGCTTGTGAC	55.0	50.0	58.0	58.0	1817-1873 bp	1.0	86.4