Hotspot

ycf4

LSC · 65154-65708 · 555 bp

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Pi 0.0018

MarkerSeek score 30.00

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 3
pi: 0.0018
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	GCAATTGCGATGGCTTCTTT	CAAGTCATCGAGCCCATGAA	45.0	50.0	58.0	58.0	1331-1547 bp	1.0	84.8
ycf4_p2	GCAATTGCGATGGCTTCTTT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.0	58.1	1492-1708 bp	1.0	84.3
ycf4_p3	GCAATTGCGATGGCTTCTTT	AGAGTCCACTCCAAATTCGC	45.0	50.0	58.0	57.9	1236-1451 bp	1.0	84.2
ycf4_p4	GATGCAGTTTCCCCTGAACA	CAAGTCATCGAGCCCATGAA	50.0	50.0	58.1	58.0	1183-1403 bp	1.0	84.0
ycf4_p5	TGATGCAGTTTCCCCTGAAC	CAAGTCATCGAGCCCATGAA	50.0	50.0	58.1	58.0	1184-1404 bp	1.0	84.0