Hotspot

ycf1

SSC · 129805-135480 · 5676 bp

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Pi 0.00767

MarkerSeek score 39.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5676
variable_sites: 121
pi: 0.00767
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCCACTTTCTTCAGTTGCT	ACTTCCGAAACAAAGGGGAC	50.0	50.0	58.0	58.0	1186-1189 bp	1.0	82.0
ycf1_p2	CTGCCCCACTTTCTTCAGTT	ACTTCCGAAACAAAGGGGAC	50.0	50.0	58.0	58.0	1189-1192 bp	1.0	82.0
ycf1_p3	CCCCACTTTCTTCAGTTGCT	GGGAAAGCGAGGAAGAAACA	50.0	50.0	58.0	58.1	1224-1227 bp	1.0	81.3
ycf1_p4	CCCCACTTTCTTCAGTTGCT	TGAAATGGGTTGGATTGGCA	50.0	45.0	58.0	58.0	1416 bp	0.357143	54.2
ycf1_p5	CTGCCCCACTTTCTTCAGTT	TGAAATGGGTTGGATTGGCA	50.0	45.0	58.0	58.0	1419 bp	0.357143	54.2