Hotspot

ndhA

SSC · 125602-127795 · 2194 bp

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Pi 0.00613

MarkerSeek score 42.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2194
variable_sites: 40
pi: 0.00613
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGCAGCTCGTACTGTTTGTT	AGCTCCAAAGGGTGAATTGG	45.0	50.0	58.1	58.1	2523-2579 bp	1.0	81.0
ndhA_p2	TTGCAGCTCGTACTGTTTGT	AGCTCCAAAGGGTGAATTGG	45.0	50.0	58.1	58.1	2524-2580 bp	1.0	81.0
ndhA_p3	TGCAGCTCGTACTGTTTGTT	TGGGAAGTCCAATGGCAAAA	45.0	45.0	58.1	57.9	2777-2833 bp	1.0	80.6
ndhA_p4	TTGCAGCTCGTACTGTTTGT	TGGGAAGTCCAATGGCAAAA	45.0	45.0	58.1	57.9	2778-2834 bp	1.0	80.6
ndhA_p5	ACGACCCAAGGCAATTTGAT	AGCTCCAAAGGGTGAATTGG	45.0	50.0	58.1	58.1	2898-2954 bp	1.0	80.5