Hotspot

psaJ-rpl33

LSC · 72754-73231 · 478 bp

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Pi 0.00361

MarkerSeek score 38.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 5
pi: 0.00361
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCCGGATGCGTTGATATTC	AACATCTTTACCCTTGGCCA	50.0	45.0	57.8	57.0	506-531 bp	1.0	75.5
psaJ-rpl33_p2	CCCGGATGCGTTGATATTCT	AACATCTTTACCCTTGGCCA	50.0	45.0	57.8	57.0	505-530 bp	1.0	75.5
psaJ-rpl33_p3	TCCCGGATGCGTTGATATTC	GAACATCTTTACCCTTGGCCA	50.0	47.6	57.8	58.2	507-532 bp	1.0	73.9
psaJ-rpl33_p4	CCCGGATGCGTTGATATTCT	GAACATCTTTACCCTTGGCCA	50.0	47.6	57.8	58.2	506-531 bp	1.0	73.8
psaJ-rpl33_p5	TCCCGGATGCGTTGATATTC	CGAACATCTTTACCCTTGGC	50.0	50.0	57.8	56.8	508-533 bp	1.0	73.8