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psbE-petL

LSC · 70080-71401 · 1322 bp

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Pi 0.00717

MarkerSeek score 56.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1322
variable_sites: 25
pi: 0.00717
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AACCGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.6	1391-1420 bp	1.0	69.2
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	ACCGCTAGTAGAAAACCGAA	50.0	45.0	57.8	56.6	1390-1419 bp	1.0	69.2
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AACCGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.6	1477-1506 bp	1.0	69.0
psbE-petL_p4	ACGAATAACCAACCCGCAAT	ACCGCTAGTAGAAAACCGAA	45.0	45.0	58.2	56.6	1476-1505 bp	1.0	69.0
psbE-petL_p5	TGCTGACGAATAACCAACCC	ACCGCTAGTAGAAAACCGAA	50.0	45.0	58.2	56.6	1481-1510 bp	1.0	68.9