Hotspot

ndhC-trnV-UAC

LSC · 55787-56347 · 561 bp

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Pi 0.00913

MarkerSeek score 60.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 12
pi: 0.00913
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCCGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	659-692 bp	1.0	82.7
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	671-704 bp	1.0	81.1
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCCGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	658-691 bp	1.0	80.0
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCCGAAAT	CGGTTCGAGTCCGTATAGTC	50.0	55.0	57.9	57.3	649-682 bp	1.0	79.0
ndhC-trnV-UAC_p5	CCTTTGCTAATCGGGGCTAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	670-703 bp	1.0	78.3