Hotspot

trnF-GAA-ndhJ

LSC · 53236-54075 · 840 bp

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Pi 0.00625

MarkerSeek score 55.50

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 13
pi: 0.00625
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AATGCCGGAAAGCTGGATAG	55.0	50.0	58.0	58.0	968-1001 bp	1.0	84.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AATGCCGGAAAGCTGGATAG	50.0	50.0	58.1	58.0	932-965 bp	1.0	84.5
trnF-GAA-ndhJ_p3	TGATACGCCAGAAATGGTCG	AATGCCGGAAAGCTGGATAG	50.0	50.0	58.1	58.0	987-1020 bp	1.0	84.1
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GAAAGCTGGATAGGATGGCC	55.0	55.0	58.0	58.1	961-994 bp	1.0	84.1
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GGAAAGCTGGATAGGATGGC	55.0	55.0	58.0	58.1	962-995 bp	1.0	84.1