Hotspot

lhbA-trnG-UCC

LSC · 40458-40830 · 373 bp

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Pi 0.0184

MarkerSeek score 55.00

Primer available yes

Label lhbA-trnG-UCC

Feature Metadata

label_name: lhbA-trnG-UCC
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 16
pi: 0.0184
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
lhbA-trnG-UCC_p1	CTTCTCCTGATGGTTGGTCG	ATTGAACCCGCATCTTCTCC	55.0	50.0	58.0	58.0	531-665 bp	1.0	82.7
lhbA-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	ATTGAACCCGCATCTTCTCC	50.0	50.0	57.8	58.0	534-668 bp	1.0	81.2
lhbA-trnG-UCC_p3	TCCTGATGGTTGGTCGAGTA	ATTGAACCCGCATCTTCTCC	50.0	50.0	57.8	58.0	527-661 bp	1.0	81.1
lhbA-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	ATTGAACCCGCATCTTCTCC	55.0	50.0	58.3	58.0	532-666 bp	1.0	80.8
lhbA-trnG-UCC_p5	TTCTCCTGATGGTTGGTCGA	ATTGAACCCGCATCTTCTCC	50.0	50.0	58.4	58.0	530-664 bp	1.0	80.0