Hotspot

psbC-trnS-UGA

LSC · 39526-39773 · 248 bp

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Pi 0.0205
MarkerSeek score 53.90
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
248
variable_sites
10
pi
0.0205
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TTATGGCATGCAGGAAGAGC GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.3 58.1 350-424 bp 1.0 82.9
psbC-trnS-UGA_p2 GCTGCAGCAGGATTTGAAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 57.6 58.1 323-397 bp 1.0 82.1
psbC-trnS-UGA_p3 TTATGGCATGCAGGAAGAGC GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.3 58.2 296-370 bp 1.0 82.0
psbC-trnS-UGA_p4 GCTGCAGCAGGATTTGAAAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.6 58.2 269-343 bp 1.0 81.1
psbC-trnS-UGA_p5 TTATGGCATGCAGGAAGAGC TGGTTGATAGCTCCGGTCTT 50.0 50.0 58.3 58.4 351-425 bp 1.0 80.0