Hotspot

trnS-GCU-trnG-GCC

LSC · 9406-10225 · 820 bp

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Pi 0.011
MarkerSeek score 50.80
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
820
variable_sites
24
pi
0.011
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA TGGAACGAATCACACTTTTACC 50.0 40.9 58.0 56.9 864-904 bp 1.0 60.8
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC TGGAACGAATCACACTTTTACC 50.0 40.9 58.0 56.9 863-903 bp 1.0 60.8
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA GGAACGAATCACACTTTTACCA 50.0 40.9 58.0 56.9 863-903 bp 1.0 60.7
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC GGAACGAATCACACTTTTACCA 50.0 40.9 58.0 56.9 862-902 bp 1.0 60.7
trnS-GCU-trnG-GCC_p5 ACAAAAGCGGAAAGAGAGGG GGAACGAATCACACTTTTACCA 50.0 40.9 58.1 56.9 923-963 bp 1.0 59.6