Hotspot

rps16-trnQ-UUG

LSC · 6465-7965 · 1501 bp

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Pi 0.00695

MarkerSeek score 53.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1501
variable_sites: 30
pi: 0.00695
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AATGGCGGATGTCAGAATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1611-1697 bp	1.0	81.6
rps16-trnQ-UUG_p2	CAGAATCCACAACCGACCAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1599-1685 bp	1.0	80.9
rps16-trnQ-UUG_p3	TCCACAACCGACCATATCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1594-1680 bp	1.0	80.8
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1572-1658 bp	1.0	80.8
rps16-trnQ-UUG_p5	AATGGCGGATGTCAGAATCC	TGGCTAAGTGGTAAGGCAAC	50.0	50.0	58.0	57.8	1652-1738 bp	1.0	80.6