Hotspot

rps7

Genome · 1104-1571 · 468 bp

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Pi 0.00641
MarkerSeek score 48.20
Primer available yes
Label rps7

Feature Metadata

label_name
rps7
feature_type
gene
region
Genome
length_bp
468
variable_sites
3
pi
0.00641
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7_p1 AATCAAGGGCGTTCTAGTGC AGGCATTCCTGTGCATTCAA 50.0 45.0 58.3 58.1 1504 bp 1.0 78.1
rps7_p2 GCAATCAAGGGCGTTCTAGT AGGCATTCCTGTGCATTCAA 50.0 45.0 58.3 58.1 1506 bp 1.0 78.1
rps7_p3 AATCAAGGGCGTTCTAGTGC ACCAGGAAAAACCATACGCA 50.0 45.0 58.3 57.7 1730 bp 1.0 76.3
rps7_p4 GCAATCAAGGGCGTTCTAGT ACCAGGAAAAACCATACGCA 50.0 45.0 58.3 57.7 1732 bp 1.0 76.3
rps7_p5 AAACCCAATTCCGCCTTACG AGGCATTCCTGTGCATTCAA 50.0 45.0 58.8 58.1 1705 bp 1.0 73.4