Hotspot

trnR-ACG-5S

Genome · 109870-110009 · 140 bp

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Pi 0.0299
MarkerSeek score 69.80
Primer available yes
Label trnR-ACG-5S

Feature Metadata

label_name
trnR-ACG-5S
feature_type
igs
region
Genome
length_bp
140
variable_sites
4
pi
0.0299
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-5S_p1 TGAAACTCTGAGGCTGGTTG CCCACGGAAAAATAGCTCGA 50.0 50.0 57.7 58.0 257-263 bp 1.0 80.8
trnR-ACG-5S_p2 TGTTGAAACTCTGAGGCTGG CCCACGGAAAAATAGCTCGA 50.0 50.0 57.7 58.0 260-266 bp 1.0 80.8
trnR-ACG-5S_p3 GTTGAAACTCTGAGGCTGGT CCCACGGAAAAATAGCTCGA 50.0 50.0 57.7 58.0 259-265 bp 1.0 80.8
trnR-ACG-5S_p4 TGAAACTCTGAGGCTGGTTG AATCCATCCCGAACTTGGTG 50.0 50.0 57.7 57.9 315-321 bp 1.0 79.4
trnR-ACG-5S_p5 TGAAACTCTGAGGCTGGTTG CAATCCATCCCGAACTTGGT 50.0 50.0 57.7 57.9 316-322 bp 1.0 79.4