Hotspot

trnN-GUU-trnR-ACG

Genome · 109195-109795 · 601 bp

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Pi 0.0207
MarkerSeek score 67.40
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
Genome
length_bp
601
variable_sites
12
pi
0.0207
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 TTCCTCAGTAGCTCAGTGGT TACAGATCAAGTAGGGGCGG 50.0 55.0 57.7 58.6 767-1084 bp 1.0 75.6
trnN-GUU-trnR-ACG_p2 GGCTGTTAACCGATTTGTCG TACAGATCAAGTAGGGGCGG 50.0 55.0 57.5 58.6 738-1055 bp 1.0 74.1
trnN-GUU-trnR-ACG_p3 TTCCTCAGTAGCTCAGTGGT CTCAGTGGAGTAGAGCACGT 50.0 55.0 57.7 58.8 738-1055 bp 1.0 73.8
trnN-GUU-trnR-ACG_p4 GGCTGTTAACCGATTTGTCG CTCAGTGGAGTAGAGCACGT 50.0 55.0 57.5 58.8 709-1026 bp 1.0 72.2
trnN-GUU-trnR-ACG_p5 TTCCTCAGTAGCTCAGTGGT TCAGTGGAGTAGAGCACGTG 50.0 55.0 57.7 59.1 737-1054 bp 1.0 71.5