Hotspot

ndhE-ndhG

Genome · 96559-96660 · 102 bp

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Pi 0.0297
MarkerSeek score 69.70
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
Genome
length_bp
102
variable_sites
3
pi
0.0297
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 TGCTCTAACCATATTTCTACTCGT AGCTCGTGGAGAACAAGTAA 37.5 45.0 57.3 56.5 235 bp 1.0 41.4
ndhE-ndhG_p2 TGCTCTAACCATATTTCTACTCGT TTAGCTCGTGGAGAACAAGT 37.5 45.0 57.3 56.5 237 bp 1.0 41.4
ndhE-ndhG_p3 GTGCTCTAACCATATTTCTACTCG AGCTCGTGGAGAACAAGTAA 41.7 45.0 57.0 56.5 236 bp 1.0 41.4
ndhE-ndhG_p4 TGCTCTAACCATATTTCTACTCGTT AGCTCGTGGAGAACAAGTAA 36.0 45.0 57.9 56.5 235 bp 1.0 41.4
ndhE-ndhG_p5 GTGCTCTAACCATATTTCTACTCG TTAGCTCGTGGAGAACAAGT 41.7 45.0 57.0 56.5 238 bp 1.0 41.3