Hotspot

trnE-UGA-trnT-GGU

Genome · 37260-37629 · 370 bp

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Pi 0.0227
MarkerSeek score 64.20
Primer available yes
Label trnE-UGA-trnT-GGU

Feature Metadata

label_name
trnE-UGA-trnT-GGU
feature_type
igs
region
Genome
length_bp
370
variable_sites
8
pi
0.0227
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UGA-trnT-GGU_p1 GTCGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.0 58.0 455-473 bp 1.0 84.4
trnE-UGA-trnT-GGU_p2 TCGCCTCCTTGAAAGAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 58.1 58.0 454-472 bp 1.0 83.8
trnE-UGA-trnT-GGU_p3 GTCGCCTCCTTGAAAGAGAG CCGATGACTTACGCCTTACC 55.0 55.0 58.0 58.2 452-470 bp 1.0 83.3
trnE-UGA-trnT-GGU_p4 TCGCCTCCTTGAAAGAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 58.1 58.2 451-469 bp 1.0 82.7
trnE-UGA-trnT-GGU_p5 GAAAGAGAGATGTCCTGGGC GAACCGATGACTTACGCCTT 55.0 50.0 57.7 58.0 444-462 bp 1.0 82.0