Hotspot

trnH-GUG-ycf2

Genome · 29942-30543 · 602 bp

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Pi 0.02
MarkerSeek score 66.50
Primer available yes
Label trnH-GUG-ycf2

Feature Metadata

label_name
trnH-GUG-ycf2
feature_type
igs
region
Genome
length_bp
602
variable_sites
12
pi
0.02
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-ycf2_p1 AAGGCAGTGGATTGTGGATC CAAATTCTTTGACAAAATTTCAGT 50.0 25.0 57.9 53.5 745-755 bp 1.0 41.5
trnH-GUG-ycf2_p2 AAGGCAGTGGATTGTGGATC TCAAATTCTTTGACAAAATTTCAG 50.0 25.0 57.9 53.3 746-756 bp 1.0 41.5
trnH-GUG-ycf2_p3 GGACGTAGCCAAGTGGTTTA CAAATTCTTTGACAAAATTTCAGT 50.0 25.0 57.5 53.5 764-774 bp 1.0 41.5
trnH-GUG-ycf2_p4 GCCAAGTGGTTTAAGGCAGT CAAATTCTTTGACAAAATTTCAGT 50.0 25.0 58.7 53.5 757-767 bp 1.0 41.5
trnH-GUG-ycf2_p5 GGACGTAGCCAAGTGGTTTA TCAAATTCTTTGACAAAATTTCAG 50.0 25.0 57.5 53.3 765-775 bp 1.0 41.5