Hotspot

atpF

LSC · 11904-13188 · 1285 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1285
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGCGCGAATGGTTACCATAA	GGGCGAAGTGATCCAAAAAG	45.0	50.0	57.7	57.4	1418 bp	1.0	72.4
atpF_p2	CAGCGCGAATGGTTACCATA	GGGCGAAGTGATCCAAAAAG	50.0	50.0	58.4	57.4	1419 bp	1.0	71.5
atpF_p3	AGCGCGAATGGTTACCATAA	GGGGCGAAGTGATCCAAAAA	45.0	50.0	57.7	58.7	1419 bp	1.0	71.5
atpF_p4	ATCGCTCAATACGTTCACGG	GGGCGAAGTGATCCAAAAAG	50.0	50.0	58.4	57.4	1458 bp	1.0	71.4
atpF_p5	CGCTCAATACGTTCACGGAT	GGGCGAAGTGATCCAAAAAG	50.0	50.0	58.4	57.4	1456 bp	1.0	71.4